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BTI Cell Growth Factors & Attachment Factors BTI has been manufacturing growth factor (GF) and attachment factor (AF) products since 1981. Our extensive product line includes receptor-grade, aseptically packaged GFs and AFs. These are highly purified proteins that have been membrane filtered, aseptically filled and lyophilized. Most BTI GFs have been tested in cell culture, receptor binding studies and in immunoassays. Some GFs are available [I125] labelled. Please consult other sections of our web site for growth factor antibodies and immunoassays. We also make available Cell Culture Additives & Components. For more information about the products, click on the name of the product.
Abbreviations: AF = Aseptically
Filled
* St. Sol. = Stabilized
Solution EGF Receptor Grade Epidermal Growth Factor (Receptor Grade) is isolated from mouse submaxillary glands and is purified to electrophoretic homogeneity. Each lot is analyzed for radioreceptor binding, DNA replication (cell culture), immunological activity via RIA, and absolute purity via HPLC. EGF (Receptor Grade) is membrane filtered and packaged as a lyophilized solid. Mouse
EGF, Receptor Grade anti-Mouse
EGF Human EGF BTI Human Epidermal Growth Factor (HEGF) is recombinant DNA produced from E. Coli. It is structurally identical (53 amino acids) to the natural Human EGF. Each lot is analyzed for radioreceptor binding, DNA replication (cell culture), immunological activity via RIA and absolute purity via HPLC. BTI Human EGF is membrane filtered and packaged as a lyophilized solid. Human EGF, Receptor Grade
anti-Human EGF, Rat EGF EGF from human and mouse sources is significantly different from rat EGF. Antibodies directed to human or mouse EGF do not recognize rat EGF sufficiently to adequately assay, localize or inhibit rat EGF containing samples. Receptor-binding studies on rat cells are much "cleaner" using BTI Rat EGF reagents. Rat EGF, Receptor Grade
anti-Rat EGF, ASSOCIATED PRODUCTS: Endothelial Mitogen (ECGS) Endothelial Mitogen (ECGS) is a partially purified preparation from bovine hypothalamus. Endothelial Mitogen was originally prepared to facilitate the growth and survival of human (or mammalian) vascular endothelial cells in vitro. BTI ECGS is now used to improve growth, supplement and/or reduce serum concentrations in cultures of endothelial cells, Balb/C-3T3 cells and in hybridoma cell cloning. Optimal concentration range is from 25-200ug/ml and BTI ECGS does not contain streptomycin which may be toxic to cells. ECGS is membrane filtered, packaged as a lyophilized solid and tested for endotoxins and mycoplasma. Catalog No: BT-203 References
Acidic FGF Acidic FGF, a member of the Class l heparin-binding growth factors (Lobb et al, JBC, 261, 1924, 1986) is structurally and functionally nearly identical to endothelial cell growth factor (ECGF), eye derived growth factor-II, and RDGF. The peptide has potent angiogenic activity and is mitogenic, potentiated by heparin, for vascular endothelial cells and other mesoderm-derived cells. BTI offers both bovine acidic FGF and human recombinant acidic FGF. BTI Bovine aFGF is purified to near homogeneity from bovine hypothalami using heparin-sepharose affinity chromatography (Burgess et al, JBC, 260: 11389, 1985). The BTI human aFGF (15.5kDa) is produced in E.Coli (1-140 a.a.) and purified to near homogeneity. Both products are membrane filtered and aseptically lyophilized. Acidic FGF, Bovine Acidic FGF, Human Basic FGF Basic FGF, a Class II heparin-binding growth factor is characterized by an isoelectric point 9.6 and elution from heparin-sepharose at 1.6-1.8M NaCl. bFGF has similar biological activities to aFGF, but mitogenicity is not potentiated by heparin. BTI prepares both bovine and human bFGF. BTI Bovine bFGF is purified (~95%, SDS-PAGE) from bovine pituitary glands via heparin sepharose and ion exchange chromatography. Our human bFGF (m.wt. 17,304) is produced in E.Coli and purified to near homogeneity. Both products are membrane filtered and aseptically lyophilized. Basic FGF, Bovine Basic FGF, Human anti-Basic FGF IgG BTI anti-bFGF is prepared in rabbits from human recombinant bFGF. The IgG is purified from the rabbit immune serum. It recognizes bFGF and not aFGF on Western immunoblots. Greater than 90% inhibition of bFGF (10ng/ml) was demonstrated on BAEC using this antibody at 1:50 to 1:100. bFGF has been localized in a variety of cells and tissues from human, bovine, rat and mouse sources using BTI anti-bFGF IgG. Catalog No: BT-583 References
Human IGF-I & II BTI Human IGF-I and Human IGF-II are recombinant DNA produced. Both have full biological activity and are suitable for cell culture or receptor studies. Human IGF-I Human IGF-II Nerve Growth Factor Nerve Growth Factor, 2.5S NGF, is isolated from mouse submaxillary glands. It is purified by a modification of the method of Bocchini and Angeletti (PNAS 64:787, 1969) to greater than 95% (PAGE). Our NGF is bioassayed in a rat pheochromocytoma cell line. 2.5S NGF is membrane filtered and packaged as a lyophilized solid. Catalog No: BT-206 anti-NGF BTI anti-NGF is produced in rabbits from our mouse 2.5S NGF. The resulting antibody is purified by absorption. 90% inhibition of NGF (10ng/ml) activity was demonstrated on PC-12 cells at 1:100 dilution. Western blots of mouse 2.5S NGF (5ng) are detectable at 1:200 dilution of our anti-NGF using GAR alkaline phosphatase detection. Catalog No: BT-555 Bovine Pituitary Extract BTI Bovine Pituitary Extract, BPE, is an extract prepared from bovine pituitary glands. BPE also known as Keratinocyte Growth Supplement is used in conjunction with other growth factors, hormones and media to reduce or eliminate serum in the culturing of some epithelial cell types. BPE has been effectively utilized in culturing normal human epidermal keratinocytes (Tsao, M.C., et al, J. Cell Physiol. 110: 219-229, 1982) and other epithelial cells. BTI BPE is membrane filtered and packaged as a lyophilized solid. Catalog No: BT-215 Human PdGF BB, Receptor Grade BTI Human PdGF BB is recombinant DNA produced and >97% pure via SDS gel electrophoresis. The activity of this product is similar to native h-PdGF in both receptor binding and in mitogen assays. Catalog No: BT-103 (lyophilized)
anti-Human PdGF BB, Neutralizing BTI anti-Human PdGF BB is produced in sheep from Human PdGF-B chain. The IgG is purified from immune serum. The antibody is highly specific, it recognizes only B-chain not A-chain PdGF. It is an excellent neutralizing antibody. Catalog No: BT-596 Human PdGF AA, Receptor
Grade Human TGF-alpha Human Transforming Growth Factor-alpha, TGF-alpha, is produced by recombinant DNA. Each lot (>98% Pure, HPLC) is evaluated for EGF receptor binding and in our Human TGF-alpha RIA Kit. Packaged as a lyophilized solid. Catalog No: BT-110 Human
TGF-alpha RIA Kit Human TGF-beta 1 BTI Human Transforming Growth Factor-beta1 is isolated from blood bank produced human platelets. TGF-beta1 is purified >97% (SDS PAGE). BTI TGF-beta1 is available lyophilized with BSA as carrier protein, and, it is also available in bulk as carrier-free in TFA/acetonitrile solution, (BT-213CF). Catalog No : BT-213
0.5ug [I125] TGF-beta 1, Receptor Grade BTI [I125] TGF-beta 1 is made from our Human TGF-beta 1. Each lot is analyzed for specific receptor binding on NRK-49F cells. The specific activity is 100-150uCi/ug and the product is packaged with a protein stabilizer. A complete protocol for receptor analysis is provided with this product. Catalog No: BT-213R*
*Dry ice shipment Human VEGF
Human Vascular Endothelial Cell Growth Factor, h-VEGF, is an angiogenesis factor produced by vascularized tissue. It is unique in that its activity is specific for endothelial cells and it has vascular permeability activity. BTI VEGF (38kDa) is a recombinant DNA produced dimer (>98%, SDS PAGE) consisting of two 165 amino acid chains covalently linked via disulfide bond. Each lot is evaluated on capillary endothelial cells for biological activity (6-10ng/ml) and in a receptor binding assay with [I125] VEGF. BTI VEGF is packaged as a lyophilized solid. Catalog No: BT-112 BTI Biomatrix I
BTI Biomatrix I is prepared from mouse EHS tumor. It is the solubilized tumor tissue basement membrane containing collagen type IV, laminin, heparin sulfate proteoglycan and other extra-cellular components. It can be used diluted (approximately 5ug/cm2) or undiluted to form a gel. Each lot of BTI Biomatrix I is analyzed for the attachment and differentiation of PC12 cells in long-term culture. It is conveniently packaged in 1ml aliquots at 20-25 milligrams [protein] per ml. Catalog No: BT-280 *Dry
ice shipment Fibronectin Fibronectin is a major cell
surface glycoprotein and is also present in blood plasma. Fibronectins
are used to facilitate the attachment of cells to a variety of surfaces
and to maintain cells in low serum conditions. Fibronectin from three
different species are purified from plasma to 95% (5% SDS PAGE) and provided
as a lyophilized solid.The BTI Human Fibronectin EIA and Elisa Kits
can effectively measure levels of human fibronectin to 50 nanograms per
milliliter (Standards are available for other species, please inquire).
*Membrane filtered and aseptically
lyophilized. Stabilized Fibronectin BTI has developed stabilized fibronectin solutions. Many lyophilized fibronectin preparations are difficult to solubilize (recoveries are only 85-95% at best) and after solubilization these preparations tend to aggregate and precipitate on standing. The BTI Fibronectin Solutions are completely solubilized and are stabilized to prevent precipitation. They retain full biological activity and are stable for 9 months stored at 4oC. The BTI Fibronectin Solutions are convenient for tissue culture and can be used whenever accurately known quantities of fibronectin are necessary. Human, Bovine and Rat Fibronectin Solutions are available at 1mg/ml, packaged aseptically following membrane filtration. Human Fibronectin, Stabilized
Bovine Fibronectin, Stabilized
Rat Fibronectin, Stabilized
Collagen Type I, (Rat Tail) BTI Rat Tail Collagen is prepared aseptically from rat tail tendons. Each lot is evaluated in cell culture using primary neuron superior cervical ganglia and PC-12 cells. The typical protein concentration is 1.5-2.2mg/ml. Catalog No: BT-274 Reference
Laminin BTI Laminin is purified (>95%) from mouse EHS sarcoma. Laminin (1-10ug/cm2) is useful in the attachment and spreading of epithelial, neuronal and endothelial cells. Laminin must be stored and shipped frozen. At room temperature, it forms a gel. Catalog No: BT-276 anti-Laminin IgG BTI anti-Laminin is produced in rabbits from mouse laminin. The antibody is purified by adsorption and is sold as an IgG fraction. It is suitable for immunohistochemical localization studies on frozen or paraffin sections, Elisa and immunoblots. Catalog No: BT-594 Poly-D-Lysine BTI Poly-D-Lysine (30,000-70,000) is a synthetic peptide used to promote cell attachment. It is membrane filtered and aseptically lyophilized. Approximately 2ug of Poly-D-Lysine per square centimeter of plating surface is recommended. Catalog No: BT-227 Other Products Human
Fibronectin Elisa Kit anti-Human
Fibronectin IgG (rabbit) anti-Human
Fibronectin (rabbit) anti-Mouse
Fibronectin (rabbit) |
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