BTI immunoassays provide you with a simple, highly specific methodology to accurately measure your samples. Both ELISA and RIA kits (& component systems) are available. The ELISA kits are provided in a 96-well (strip) format and the RIA kits are sold on a tube basis.

BTI manfactures 7 different assays for Osteocalcin. Some assays are suitable for more than one species of Osteocalcin. Click here to see the chart of different species and the assays that can be used.

A complete protocol is provided with each kit or component system. For more complete information, please contact us.

For more information about the products, click on the name of the product.
 

ELISA Kits	Cat. #	Range	Sample Size	Kit Size	Price/Kit
Cyclic AMP	BT-730	0.05-100pm/ml	100ul	2x96 wells	$310.00
Cyclic GMP	BT-740	0.05-100pm/ml	100ul	2x96 wells	$310.00
Human EGF	BT-720	0.5-100ng/ml	50ul	1x96 wells	$345.00
Human Fibronectin	BT-700	25-2000ng/ml	100ul	1x96 wells	$350.00
Human Involucrin	BT-650	0.5-10ng/ml	5-100ul	2500 wells	$750.00
Human Lysozyme	BT-630	0.78-50ng/ml	100ul	1x96 wells	$365.00
Intact Osteocalcin	BT-460	1.0-50ng/ml	25ul	1x96 wells	$385.00
Mid-Tact Osteocalcin	BT-480	1.0-50ng/ml	25ul	1x96 wells	$345.00
Rat Osteocalcin	BT-490	0.3-20ng/ml	100ul  Serum 1:20	1x96 wells	$385.00
Mouse Osteocalcin	BT-470	1.0-50ng/ml	25ul  Serum 1:10 to 1:20	1x96 wells	$385.00

 

RIA Kits	Cat. #	Range	Sample Size	Kit Size	Price/Kit
Cyclic AMP	BT-300	0.05-200pm/ml	100ul	200T	$310.00
Cyclic GMP	BT-340	0.05-200pm/ml	100ul	200T	$310.00
Human Osteocalcin	BT-440	1.0-50ng/ml	50ul	100T	$345.00
Mouse Osteocalcin	*	1.5-200ng/ml	50ul  Serum 1:50	*Component  System	.
Rat Osteocalcin	*	0.1-100ng/ml	100ul  Serum 1:50	*Component  System	.
Human TGF-alpha	BT-350	0.2-50ng/ml	100ul	100T	On Request

*Complete Component Systems are Available, please inquire.

Cyclic AMP & Cyclic GMP EIA Kits

About The Assays

The BTI Cyclic AMP and Cyclic GMP EIA Kits are complete systems.  Each kit uses alkaline phosphatase labeled tracer (cAMP or cGMP) competing for sites on the antibody with standard or sample.  Both assays employ a highly specific antibody developed by BTI to insure accurate, consistent results.  The assay sensitivities are outstanding.  As little as 0.005 picomoles per well of cAMP or cGMP can be measured.

Cyclic AMP EIA Kit Range
0.5-100 picomoles/ml
0.05-10 picomoles/ml (acetylated)

Cyclic GMP EIA Kit Range
0.5-100 picomoles/ml
0.05-10 picomoles/ml (acetylated)

Both kits include all the necessary reagents (plus two coated 96-well strip ELISA plates) to perform the tests either acetylated or unacetylated.  Levels of cAMP or cGMP can be conveniently measured from most biological samples (urine, plasma and cell culture) without the extensive purification procedures inherent in other methodologies.

These are the most versatile cAMP and cGMP kits available. Different sample types can be accommodated easily ( ie. Cell culture supernates and cell extracts(~p[H] 7) ) without sacrificing specificity or sensitivity. BTI scientists have extensive experience in working with a large variety of samples and are available for consultation.

Both the Cyclic AMP and Cyclic GMP assays can be run either overnight or in 6 hours.  The protocol includes only two pipeting steps and one wash sequence per assay.  Each kit accommodates 80 samples run in duplicate (or 160 wells for unknowns).

The shelf life of these BTI Cyclic EIA Kits is 5 months stored at 4ºC.

Procedural Outline
(for both Cyclic AMP and Cyclic GMP EIA Kits)

1. Add samples/standards, tracer and antibody to appropriate wells.  Cover and
    incubate at 4ºC overnight or at 20ºC for 4 hours.

2. Aspirate and wash five times.

3. Blot excess liquid.  Add 200ul substrate to all wells, cover, incubate for 2 hours at 37ºC.
    Measure absorbance at 410nm.

BTI Cyclic AMP EIA Kit
(sufficient reagents and 2 precoated 96-well strips)
Catalog No:  BT-730
Quantity:  1 kit

BTI Cyclic GMP EIA Kit
(sufficient reagents and 2 precoated 96-well strips)
Catalog No:  BT-740
Quantity:  1 kit
 

Cyclic AMP EIA Kit Specifications: Sample Size:  100ul Assay Time:  6 hours or overnight Sensitivity:  .05 pmoles/ml Range:  0.05-100 pmoles/ml Intraassay Variation:  3% Interassay Variation:  8%

Cyclic GMP EIA Kit Specifications: Sample Size:  100ul Assay Time:  6 hours or overnight Sensitivity:  .05 pmoles/ml Range:  0.05-100 pmoles/ml Intraassay Variation:  4% Interassay Variation:  8%

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

Human EGF EIA Kit

About The Test

The BTI Human EGF EIA Kit employs a competitive binding technique in which biotinylated hEGF competes with unlabelled hEGF for a limited number of specific binding sites on the immobilized antibody.  The protocol is 3.5 hours and requires only a 50ul sample.  The assay range is 0.5-100ng/ml.  Urine samples can be assayed directly without dilution.  All the necessary reagents, a human urine control, and a complete protocol are included with the kit.
 

BTI Human EGF EIA Kit
Catalog No:  BT-720
(sufficient reagents and precoated 96-well strips)
Quantity:  1 kit

Refrigerate at 4ºC.

Specifications:
Sample Size:  50ul
Assay Time:  3.5 hours
Sensitivity:  0.5 ng/ml
Range:  0.5 -100 ng/ml
Intraassay Variation:  5%
Interassay Variation:  7%

Expected Range in normal urine: 5 - 50ng/ml

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

Human Fibronectin Elisa Kit

Introduction

Fibronectin, a major cell surface glycoprotein, is found on cell surfaces and circulating in plasma.  It has been shown to be important in wound healing and in cellular interactions.

The BTI Human Fibronectin ELISA Kit measures human fibronectin from both plasma and cellular extracts.  The assay employs a standard 3.5 hour Elisa protocol.  A 100 microliter sample is required and the range is 25-2000 nanograms of human fibronectin per ml.

A number of other species of fibronectin have been evaluated with the kit.  The range of these other species' assays is less sensitive.  Bovine Fibronectin is 0.5-100ug/ml, Mouse Fibronectin is 0.2-50ug/ml, and Rat Fibronectin is 0.2-20ug/ml.
(Please inquire about the availability of the species you are interested in).

The kit is in a 96-well (precoated strip) format and includes all the necessary reagents and a complete protocol to perform
the assay.

References

1. Hess L.W., O’Brien W.F., Holmberg J.A., Winkel C.A., Monaghan W.P., and Hemming V.G. Plasma and Amniotic Fluid Concentrations of Fibronectin During Normal Pregnancy. Obstetrics & Gynecology, 68: 25-28 (1986).

2. Eielson C., Kaplan D., Mitnick M.A., Paliwal I., and Insogna K. Estrogen Modulates PTH-Induced Fibronectin Production in Human and Rat Osteoblast-like Cells. Endocrinology 135, No. 4: 1639-1644 (1994).

Procedural Outline

1. Add 100ul samples or standard in duplicate, add 50ul of Fibronectin Ab. Incubate at 37ºC.

2. Add 50ul alkaline phosphatase HFN conjugate. Incubate at 37ºC for 1 hour.

3. Aspirate and wash (3x).

4. Add 200ul Substrate, incubate @37deg.C 1hour. Read Absorbance at 410nm.
 

BTI Human Fibronectin Elisa Kit
Catalog No:  BT-700
Quantity: 1 kit (96-well plate)

Please Refrigerate on Arrival. Do Not Freeze.

FOR RESEARCH USE ONLY.  NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

Human Involucrin Elisa Assay

About The Assay

Involucrin, a soluble cytoplasmic protein with a highly repetitive amino acid sequence, serves as a substrate for transglutaminase cross-linking in the formation of cornified envelopes.  Like the cross-linked envelope, involucrin expression is a specific marker for keratinocyte differentiation.  This protein is synthesized not only by cells of the epidermis and other squamous epithelia, but also in epithelia undergoing squamous metaplasia and in certain tumors, as in the lung, which display epidermoid characteristics.  In culture the growth and differentiation of keratinocytes, especially those derived from tumors, can be modulated greatly by physiological (retinoids, calcium) and toxic agents (including carcinogens).  The involucrin content of the cells is highly responsive to such treatments and thus its measurement by ELISA can serve as a convenient and sensitive indicator of the cellular response.

The BTI Involucrin ELISA Kit is designed to provide a reproducible, quantitative method of measuring involucrin. The assay is highly sensitive, 0.05-0.5  nanograms per well.  Levels of human involucrin can be directly measured after separation of cellular debris.  The kit includes highly purified Human Involucrin, anti-Human Involucrin (rabbit) and Goat anti-Rabbit IgG Alkaline Phosphatase. A complete protocol is provided with each kit.

References

1.   Walts, A.E. et al (1985) Involucrin, a marker of squamous and  urothelial  differentiation.  An immunohistochemical study on its distribution  in normal and  neoplastic tissues. J. Pathol. 145: 329-340.

 2.   Eckert, R.L. and Green, H. (1986) Structure and evolution of the  human involucrin gene. Cell 46: 583-589.

 3.   Parenteau, N.L. et al (1987) Primate Involucrins: Antigenic  relatedness and detection of multiple forms. Proc. Natl. Acad. Sci. USA 84: 7571-7575.

 4.    Rice, R. H. et al (1988) Suppression of keratinocyte differentiation in SCC-9 human squamous carcinoma cells by benzo(a)pyrene, 12-O-tetradecanoyl phorbol 13 acetate and hydroxyurea.  Carcinogenesis 9: 1885-1890.

 5.    Rubin, A. L. et al  (1989) Coordination of keratinocyte programming in human SCC-13  squamous carcinoma and normal epidermal cells. J. Cell. Physiol. 138: 208-214.

 6.    Gaido, K.W. and Maness, S.C. (1994) Regulation of Gene Expression and Acceleration   of  differentiation in Human Keratinocytes By TCDD. Toxicol, & Applied Pharm. 127: 199-208.

Procedural Outline

1. Simultaneously incubate (4ºC) overnight Involucrin in Plate No.1, and Antibody + Standards or Antibody + Unknowns
    in Plate No.2.

2. Transfer contents of Plate No.2 to Plate No.1. Incubate at room temperature 30 minutes.

3. Rinse Plate No.1, add GAR IgG Alkaline Phosphatase, incubate 1 hour at room temperature.  Rinse and
    add developing agent, incubate 1-4 hours.

4. Read Plate at 405nm.

Kit Components (1 vial each)
1. anti-Human Involucrin (rabbit)
2. Human Involucrin
3. Goat anti-Rabbit IgG Alkaline Phosphatase Conjugate.

BTI Involucrin ELISA Kit
Catalog No:  BT-650
(sufficient for 2500 tests or 25-96 well plates)
Quantity:  1 kit

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

HUMAN LYSOZYME EIA KIT

• Sandwich ELISA
• Sensitive
• Non-Radioactive
• Results in 4 ¼ Hours
• 4-6 Month Shelf Life

Lysozyme (muramidase) is an enzyme present in serum, plasma, amniotic fluid, milk, saliva, tears, urine and many other biological fluids.  Lysozyme breaks down the walls of certain bacteria and protects against bacterial infections.  Lysozyme has recently been reported as having anti-viral activity against HIV type 1.  Elevated lysozyme levels in urine and serum have been reported in many human disease states, including leukemias (FAB-M4, CMML, CML), tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, pyelonephritis, nephrosis and renal transplant rejection.

The BTI Human Lysozyme EIA Kit is a sandwich ELISA assay.  The assay employs two highly specific lysozyme antibodies.  Our MAb is immobilized in the wells and a second polyclonal antibody is used as the reporter antibody.  The assay is sensitive (0.78-50ng/ml) and specific for human lysozyme.  Each Kit includes all the necessary reagents, precoated 96-well strip plate, urine control and a complete 4 ¼ hour protocol.

References

1. Hankiewiz, J. and Swierczuk, E. 1974.  Lysozymes in Human Body Fluids.  Clinica Chemica Acta, 57:  205-209.

2. Meyor, K., Gelhorn, A., Prudden, J.F., et al. 1948.  Lysozyme Activity in Ulcerative Alimentary Diseases. American Journal of Medicine, 5:  496-502.

3. Prockup, D.J. and Davidson, W.D., 1964. A Study of Urinary and Serum Lysozyme In Patients with Renal Disease, New England Journal of Medicine, 270:  269.

4. Davis, C.S. April 5, 1971. Diagnostic Value of Muramidase.  Laboratory Medicine, 51-54.

5. Lee-Huang S, Huang PL, Sun Y, et al.  Lysozyme and RNases as anti-HIV in components in beta-core preparations of human chorionic gonadotropin.  Proc Natl Acad Sci USA 1999 (Mar 16); 96(6):  2678-2681.

Procedural Outline

1. Add 100ul standard/sample/control/blank, cover and incubate at room temperature for 2 hours.

2. Aspirate and wash three times.

3. Add 100ul of lysozyme antibody to each well, and incubate at room temperature for 1 hour.

4. Repeat Step 2, then add 100ul of DAG-HRP to each well, cover, and incubate at room temperature for 1 hour.

5. Repeat Step 2, then add 100ul of substrate to all wells, and incubate for 15 minutes in the dark at room temperature.

6. Add 100ul of stop solution, swirl and read at 450nm.
 

BTI Human Lysozyme EIA Kit
Catalog No:  BT-630
(Sufficient reagents and 1 precoated 96-well strip plate)
Quantity: 1kit

Specifications:
Sample Size:  100ul
Assay Time:  4.25 hours
Sensitivity:  0.78 ng/ml
Range:  0.78-50 ng/ml
Intraassay Variation:  5.3%
Interassay Variation:  7%

FOR RESEARCH USE ONLY.  NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

Intact Human Osteocalcin EIA Kit

About The Assay

Osteocalcin (5800 daltons) is a specific product of the osteoblast.  It is primarily deposited into the ECM of bone and only a small amount of newly synthesized osteocalcin is released directly into the circulation.  Studies suggest that there are various forms of osteocalcin in the circulation and that different antibodies detect both intact and fragments of osteocalcin.  The physiological significance of osteocalcin fragments is unclear but they may be derived from resorption, osteoblastic synthesis, systemic catabolism or all of these.  Since serum proteases may also degrade the intact osteocalcin, it is recommended that blood samples be processed quickly in the presence of protease inhibitors.

The BTI Intact Osteocalcin EIA measures only intact osteocalcin which is synthesized de novo by the osteoblast and it eliminates any interference by circulating fragments.  The assay is a sandwich EIA which employs two monoclonal antibodies directed towards the amino-and the carboxy-terminal regions of the human osteocalcin.  It recognizes only intact osteocalcin, requiring the full 49 residue protein for detection. This assay reacts with human, bovine, monkey and dog osteocalcin.

The assay is highly sensitive (Range: 1-50ng/ml)  and requires only a 25ul sample.  All the necessary reagents, a 96-well strip plate, and a complete 3½ hour protocol are included with the kit.

Procedural Outline

1. Add 25ul standard/sample.  Add antibody and gently swirl.  Incubate 2½ hours at 37^oC.

2. Aspirate and wash 3 times with wash buffer.

3. Add Strep-Av-HRP, swirl, incubate ½ hour at room temperature.

4. Repeat Step 2. Then add 100ul of substrate, swirl, develop 10 minutes in the dark.
    Add 100ul stop solution, swirl, measure absorbance at  450nm.

References

1.   Gundberg C.M., Weinstein R.S. Multiple immunoreactive forms of osteocalcin in uremic serum. J. Clin Invest 77: 1762-67, 1986.

2.   Power M.J., Gosling J.P., Fotrell P.F. Radioimmunoassay of osteocalcin with polyclonal and monoclonal antibodies. Clin Chem 35: 1408-15, 1989.

3.   Calvo M.S., Eyre D.R., and Gundberg C.M. Molecular Basis and Clinical Application of Biological Markers of Bone  Turnover. Endocrine Reviews 17 (4): 333-364, 1996.

 4.   Bodine, P.V.N., Trailsmith, M. and B.S. Komm. Development and Characterization of a Conditionally Transformed Adult Human Osteoblastic Cell Line.  J. of Bone & Miner. Res. 11: 806-819, 1996.
.
 5.   Nutall, M.E., Patton, A. J., Olivera, D.L. Nadeau, D.P., &  M.  Gowen.  Human Trabecular Bone Cells are able to Express Both Osteoblastic and Adipocytic Phenotype:  Implications for Osteopenic Disorders. J. of Bone & Miner. Res. 13: 371-382,  1998.

BTI Intact Osteocalcin EIA Kit
Catalog No:  BT-460
(sufficient reagents and precoated 96-well strips)
Quantity:  1 kit
 

Specifications:
Sample Size:  25ul
Assay Time:  3.5 hours
Sensitivity:  0.5 ng/ml
Range: 1.0- 50 ng/ml
Intraassay Variation:  7%
Interassay Variation:  10.5%

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.
 
 

Mid-Tact Human Osteocalcin EIA Kit

About The Assay

Osteocalcin (5800 daltons) is a specific product of the osteoblast.  Only a small amount of osteocalcin is released directly into the circulation, it is primarily deposited into the bone matrix.  Studies have shown that osteocalcin circulates both as the intact (1-49) protein and as N-terminal fragments.  The major N-terminal fragment is the peptide (1-43).

The BTI Mid-Tact Osteocalcin Elisa Kit is highly specific. It measures both the intact human osteocalcin and the major (1-43) fragment.  The assay is a sandwich EIA which employs two monoclonal antibodies.  One antibody (1-19) is immobilized in the wells and the second antibody (30-40) is biotinylated.  The assay is highly sensitive (0.5ng/ml) and requires only a 25 microliter sample.  All the necessary reagents, a 96-well strip plate, and a complete 3½ hour protocol are included with the kit.

References

1.  Garnero P., Grimaux M., Seguin P. and P.D. Delmas. Characterization of Immunoreactive Forms of Human Osteocalcin in vivo and in vitro. J. of Bone & Mineral Res. 9 (2):  255-264, l994.

2.   Calvo M.S., Eyre D.R.,and Gundberg C.M.  Molecular Basis and Clinical Application of Biological Markers of Bone Turnover. Endocrine Reviews 17(4): 333-364, 1996.

Procedural Outline

1.  Add 25ul standard/sample. Add antibody and gently swirl. Incubate 2½ hours at 37^oC.

2.  Aspirate and wash 3 times with wash buffer.

3.  Add Strep-Av-HRP, swirl, incubate 30 mins. at room temperature.

4.  Repeat Step 2. Then add 100ul of substrate, swirl, develop 15 minutes in the dark.
     Add 100ul stop solution, swirl, measure absorbance at 450nm.

BTI Mid-Tact Human Osteocalcin EIA Kit
Catalog No:  BT-480
(sufficient reagents and precoated 96-well strips)
Quantity:  1 kit

Specifications:
Sample Size:  25ul
Assay Time:  3.5 hours
Sensitivity:  0.5 ng/ml
Range:  1.0-50 ng/ml
Intraassay Variation:  7%
Interassay Variation:  10%

FOR RESEARCH USE ONLY.  NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

About the Test

The BTI Rat Osteocalcin EIA Kit is a sandwich ELISA assay.  Two osteocalcin antibodies are employed, each directed toward an end of the (C or N terminal) osteocalcin molecule.  The N-terminal monoclonal antibody is bound to the wells and when reacted with rat osteocalcin standard or sample, the C-terminal rat osteocalcin antibody completes the sandwich.  This ELISA kit shows good  correlation (r = 0.95) with our rat osteocalcin RIA.

This sandwich ELISA kit is specific for rat osteocalcin only.  Both carboxylated and decarboxylated rat osteocalcin are recognized. Rat osteocalcin can be measured directly from serum, heparinized plasma, bone extracts and serum-free cell culture supernates.

The assay can be run either overnight (0.33-20ng/ml) or in 6 hours (0.78-50ng/ml).  As little as 10 microliters of rat serum is required for duplicate determinations.  Each kit contains all the necessary reagents, an authentic rat serum control, precoated 96-well strips and a complete protocol.

BTI Rat Osteocalcin EIA Kit
Catalog No:  BT-490
(sufficient reagents and precoated 96-well strips)
Quantity : 1 kit

Specifications:
Sample Size:  100ul (diluted serum)
Assay Time:  5 hours or overnight
Sensitivity:  0.5 ng/ml
Range:  0.78-50 ng/ml or 0.33-20 ng/ml
Intraassay Variation:  4%
Interassay Variation:  7%

FOR RESEARCH USE ONLY.  NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

Human Osteocalcin RIA Kit

About The Assay

Osteocalcin, the major non-collagenous protein of bone, is a specific product of the osteoblast and its synthesis is stimulated by 1, 25-Dihydroxyvitamin D3. A fraction of that synthesized does not accumulate in bone but is secreted directly into the circulation.  Animal studies indicate serum osteocalcin is derived from new cellular synthesis rather than from the release of bone matrix protein during resorption. Furthermore, serum osteocalcin concentrations are related to histomorphometric measurements of bone formation but not resorption.  Deviations from normal concentrations of circulating osteocalcin are a consequence of changes in the synthesis or degradation of the protein.  Such changes may result from physiological alterations in skeletal homeostasis that accompany normal development or may be associated with specific disease states.  While the precise biological function of osteocalcin is not known, it is the most specific marker of osteoblastic function described
to date (1).

The availability of commercial immunoassay kits and reagents has resulted in a large body of literature regarding serum osteocalcin measurements in normal individuals and in those with a variety of metabolic bone diseases. Unfortunately, the absolute values obtained with these kits deviate substantially (2) from results obtained in various research laboratories.
The BTI Human Osteocalcin RIA Kit offers the advantages of the use of all human reagents and values that are consistent
with those obtained in research laboratories.

The BTI Human Osteocalcin RIA Kit is user friendly.  The kit includes 5 individual standards and a simplified 3.5 hour protocol.  The assay requires only a 50ul sample and yields a range of 1.0-50 nanograms per ml.  Serum samples and cell culture supernatants can be measured directly.

References

1. Hauschka P.V., Lian J.B., Cole D.E.C., Gundberg C.M. Osteocalcin and Matrix Gla Protein: Vitamin K-dependent proteins in bone. Physiological Reviews, 69: 990-1047 (1989).

2.  Delmas, P.D., Christiansen C., Mann K.G. and Price P.A. Bone Gla Protein (Osteocalcin) assay standardization report. Journal of  Bone and Mineral Research, 5:  5-11 (1990).

Procedural Outline

1. Add 50ul standard/sample. Add 100ul each antibody and [I¹²⁵] Human Osteocalcin. Incubate 2½ hours at 37^oC.

2. Add 100ul each 2nd antibody and precipitating reagent.  Incubate 10 minutes at room temperature.

3. Add 1ml buffer.  Centrifuge 15 minutes at  1800 rpm.

4. Decant or aspirate.  Count pellets in gamma counter.

BTI Human Osteocalcin RIA Kit
Catalog No:  BT-440
(sufficient reagents for 100 tubes)
Quantity:  1 kit

Specifications:
Sample Size:  50ul
Assay Time:  3.5 hours
Sensitivity:  1.0 ng/ml
Range:  1.0-50 ng/ml
Intraassay Variation:  6% (95% limits)
Interassay Variation:  9% (95% limits)

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

Mouse Osteocalcin RIA Reagents

About The Assay:

The BTI Mouse Osteocalcin RIA Reagents provide a complete RIA system to measure mouse osteocalcin.
The assay is highly sensitive, 0.2-5 nanograms per tube.  It is possible to quantitate mouse osteocalcin in as
little as 1-5 microliters of mouse serum.  A complete protocol is provided with the individual reagents.

References:

1.  Lewis, D. B. et al. PNAS (Med. Sci.)  90: 11618-11622 (1993).
2.  Wenstrup, R.J. et al. JBC 271: 10271-10276 (1996).
3.  Tsuji, H. et al. J. of Bone & Mineral Res. 11: 1234-1240 (1996).

Mouse Osteocalcin Standard
Catalog No:  BT-431
Quantity:  1 vial

Goat anti-Mouse Osteocalcin RIA Grade
Catalog No:  BT-432
Quantity: 1 vial (500 tubes)

[I¹²⁵] Mouse Osteocalcin
Catalog No:  BT-431R *
Quantity:  5uCi

Normal Goat Serum
Catalog No:  BT-415
Quantity:  5ml

Donkey anti-Goat IgG
Catalog No:  BT-414
Quantity:  5ml (sufficient for 250 tubes)

Associated Product

anti-Mouse Osteocalcin, Goat
Immunohistochemical Grade
Catalog No:  BT-592
Quantity:  0.25ml

*Dry Ice Shipment
 
 

Rat Osteocalcin RIA Reagents

About The Assay:

The BTI Rat Osteocalcin Radioimmunoassay Reagents are designed  to provide an inexpensive, flexible method for the accurate measurement of rat osteocalcin.  The assay is highly sensitive 0.025-1.5 nanograms per tube. Levels of osteocalcin can be measured directly from rat serum, bone and cell culture samples. Only 5ul of serum is required for duplicate determinations.  A complete protocol is provided with the individual reagents.

References

1. Price, P.A. Vitamins & Hormones 42, 65 (1985).
2. Gundberg, C.M. et al. Methods in Enzymology 107, 516 (1984).
3. Epstein S. et al. The Lancet 1, 307 (1984).
4. Charles, P. et al. J. Clin. Invest. 76, 2254 (1985).
5. Brown, J.P. et al. The Lancet 1, 1091 (1984).
6. Finkelman, R.D. et al. PNAS  USA 88: 3657-3660 (1991).
7. Fanti, P. et al. Am. J. Physiol. 263  (E.&M. 26): E1113-8 (1992).
8. Kimble, R.B. et al. J. Clin. Invest. 93: 1956-1967 (1994).

anti-Rat Osteocalcin (RIA Grade)
Lyophilized solid, 500 tubes/vial
Catalog No:  BT-413
Quantity:  1 vial

Rat Osteocalcin
Lyophilized solid, 99.9% Pure
Catalog No: BT-412
Quantity:  10ug

[I¹²⁵] Rat Osteocalcin
Specific Activity:  approx. 30-100uCi/ug
Catalog No:  BT-411R *
Quantity:  5uCi

Normal Goat Serum
Catalog No:  BT-415
Quantity:  5ml

Donkey anti-Goat IgG
Catalog No:  BT-414
Quantity:  5ml (sufficient for 250 tubes)

*Dry ice shipment

Human TGF-alpha RIA Kit

About The Assay

Transforming Growth Factor-alpha (TGF-alpha) is a peptide structurally related to epidermal growth factor (EGF) and also binds the EGF receptor. Synthesis of  TGF-alpha has been reported in several transformed cells and tissues during early fetal development and recently in normal adult keratinocytes.  Addition of EGF or TGF-alpha induces expression of  TGF-alpha messenger RNA, suggesting the presence of an auto-inductive mechanism in the normal regulation of the epidermis. Expression of TGF-alpha could also potentially occur during wound healing and in various pathological states besides neoplasia.

The BTI Human TGF-alpha RIA Kit employs a sensitive, highly specific methodology to measure hTGF-alpha.  The method utilizes a competitive binding technique in which [I¹²⁵] TGF-alpha compete for binding sites on our highly specific hTGF-alpha antibody.  The simple procedure involves one overnight incubation and yields a sensitivity of 0.02 nanograms.

Included in the kit are all the necessary reagents and a detailed protocol.

References

1. Wong, D.T. et al. Cancer Research 48, 3130-3134 (1988).
2. Marquardt, H. et al. Science 223, 1079-1082 (1984).
3. Derynck, R. et al. Cancer Research 47, 707-712 (1987).
4. Twardzik, D.R. Cancer Research 45, 5413-5416 (1985)
5. Coffey, R.J. et al. Nature 328, 817-820 (1987).
6. Derynck, R.J. Cell. Biochem. 32, 293-304 (1986).
7. Serwin, S.A. et al. Cancer Research 44, 3613-3619 (1983).
8. Cardinali, M. et al. J. Clin. Invest. 89 No. 2, 1242-1247 (1992).

Kit Components

1. [I¹²⁵] hTGF-alpha (1 vial, ~3uCi/vial)
2. Buffer (1 vial)
3. Standard (1 vial)
4. hTGF-alpha Antibody (1 vial)
5. Second Antibody (1 vial)
6. Precipitating Reagent (1 vial)

BTI Human TGF-alpha RIA Kit
Catalog No:  BT-350
(sufficient for 100 tubes)
Quantity:  1 kit

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.

Cyclic AMP & Cyclic GMP RIA Kits

• Sensitive
• Fast, Simple Protocol
• True N.S.B. Determinations
• Visible, Tight Pellets
• Inexpensive
• All Components Available Individually
• Improved Shelf Life

About The Test

The BTI Cyclic AMP and Cyclic GMP RIA Kits employ state-of-the-art reagents and methodologies. Both kits have highly specific primary antibodies preconjugated with a second antibody to minimize non-specific binding and to eliminate a second incubation step. The highly immunoreactive Tracers, [I¹²⁵]S-Cyclic AMP-TME and the [I¹²⁵]S-Cyclic GMP-TME are purified via HPLC and are monoiodinated.

The assay sensitivities are outstanding. The Cyclic AMP RIA Kit is 0.1 picomoles per tube and 0.005 picomoles per tube for acetylated samples. The Cyclic GMP RIA Kit is 0.2 picomoles per tube and 0.005 picomoles per tube for acetylated samples. Both kits employ a fast, simple protocol.

The protocol includes only three pipetting steps, one incubation, and generates a visible precipitate. All necessary reagents including  true non-specific binding reagents and acetylation reagents are included in each 200 tube kit. Both kits have a shelf life of 8 weeks refrigerated at 4^oC.

Levels of Cyclic AMP and Cyclic GMP can be conveniently measured from most biological samples (urine, plasma, tissue and cell culture) without the usual extensive extraction and purification  procedures inherent in other methodologies.

These are the most versatile cAMP and cGMP kits available. Different sample types can be accommodated easily ( ie. Cell culture supernates and cell extracts(~p[H] 7) ) without sacrificing specificity or sensitivity.BTI scientists have extensive experience in working with a large variety of samples and are available for consultation.

All kit components and related products are available individually for your convenience and economy. Please inquire.

References:

1. Koller, K.J. et al. Science 252, 120-123 (1991)
2. Chang, M et al. Nature 341, 68-71 (1989)
3. Finkelman, R.D. et al. Bone and Mineral Res. 16, 89-100 (1992)
4. Buczek-Thomas, J et al.  Molecular & Cellular Biochem. 145, 131-139 (1995)
5. Baylink, T.M. et al. J. of Bone and Mineral Res. 11, 1413-1418 (1996)
 

Procedural Outline (for both Cyclic AMP and Cyclic GMP RIA Kits)

1. (At room temperature) Add buffer, Standard or Sample, Tracer and Antibody to appropriate tubes.
    Mix tubes gently in rack.

2. Incubate at 4^oC overnight.

3. Add 1 ml of cold Buffer and centrifuge.

4. Decant or aspirate supernatants. Count each tube.

BTI Cyclic AMP RIA Kit (sufficient for 200 tubes)
Catalog No : BT-300
Quantity : 1 kit

Specifications:
Sample Size:  100ul
Assay Time:  overnight
Sensitivity:  0.05pmoles/ml
Range:  0.05-200pmoles/ml
Intraassay Variation:  5%
Interassay Variation:  8%

BTI Cyclic GMP RIA Kit (sufficient for 200 tubes)
Catalog No : BT-340
Quantity : 1 kit

Specifications:
Sample Size:  100ul
Assay Time:  overnight
Sensitivity:  0.05pmoles/ml
Range:  0.05-200pmoles/ml
Intraassay Variation:  5%
Interassay Variation:  7%

Standing Order discounts are available. Please inquire.

Refrigerate at 4^oC. Do Not Freeze.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMAN OR AS IN-VITRO DIAGNOSTIC.
 

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