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BTI Antibodies
BTI prepares over 35 different antibodies. Antibodies to the cytoskeletal proteins, growth & attachment factors, lipoproteins, hormones and keratinocyte differentiation markers are also offered. All our products are produced in the USA. Labeled second antibodies and precipitating antibodies are available in convenient packaging. Complete information on the effective use of each antibody is provided with the product. Most of the antibodies are provided lyophilized or in buffer containing 0.02% sodium azide. BTI antibodies are unique. They are available in North and South America, directly from BTI. We have distributors in 24 countries; Europe, Asia and Australia. For
more information about the products, click on the name of the product.
ABBREVIATIONS: ADS
= Adsorbed Transglutaminase Monoclonal Antibody Introduction The BTI Human Keratinocyte Transglutaminase MAb recognizes transglutaminase type 1 which is expressed during the differentiation of epidermis and stratified squamous epithelia. This MAb has been used for immunohistochemical localization on frozen tissues or formaldehyde (2%) fixed tissues and cells from human, hamster, monkey, rabbit and rat sources (paraffin-embedded sections do not stain properly). The BTI Transglutaminase MAb can be used in a modified elisa for the detection of type 1 transglutaminase expression in response to retinoids. This IgG2a antibody is purified via protein A sepharose. A complete protocol is included with the MAb, including methods for analyzing tissue sections and cultured cells. References 1. Phillips, M.A; Stewart, B.E; Qin, Q; Chakravarty, R; Floyd, E; Jetten, A.M; and Rice, R.H. (1990) Primary Structure of Keratinocyte Transglutaminase. Proc. Natl. Acad. Sci. 87: 9333-9337. 2. Schmidt, R; Michel, S; Shroot, B; and U. Reichert. (1988) Transglutaminase in normal and transformed human keratinocytes in culture. J. Invest. Dermatol. 90: 475-479. 3. Michel, S; Courseaux, A; Miguel, C; Bernardon, J.M; Schmidt, R; Shroot, B; Thacher, S.M; and U. Reichert (l991) Determination of retinoid activity by an enzyme-linked immunosorbent assay. Anal. Biochem. 192: 232-236. 4. Huber, M; Rettler, I; Bernasconi, K; Frenk, E; Lavrijsen, S; Ponec, M; Bon, A; Lautenschlager, D; Schorderet, D; and Hohl, D. (1995) Mutations of Keratinocyte Transglutaminase in Lamellar Ichthyosis. Science. 267: 525-527. anti-Human Keratinocyte
Transglutaminase, Mouse MAb FOR RESEARCH USE ONLY.
NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC. anti-Actin IgG 
BTI Actin Antibody is prepared in rabbits with homogeneous Actin isolated from chicken gizzards. Actin IgG is then purified from rabbit immune serum. The resulting Actin IgG binds specifically with F and G Actins from both muscle and non-muscle tissues. Invertebrate and vertebrate tissues containing Actin cross-react with BTI anti-Actin IgG. BTI anti-Actin IgG is useful for the immunohistochemical elucidation of intracellular structure and function. A complete protocol is included with each shipment including details on immunofluorescent procedures. Catalog No: BT-560 BTI anti-Cytokeratin Antibody is prepared in rabbits with pre-keratins (Citric Acid Soluble Keratins) isolated from bovine tissue. The resulting Cytokeratin Antibody is a broad spectrum antibody which will react with both acidic and basic forms of Class I & II Keratins (demonstrated by Western immunoblots of human keratinocyte homogenates). The antibody is provided as a whole serum and is excellent for the immunohistochemical staining of both tissues and cultured cells. Catalog No: BT-571 BTI anti-GFAP, Polyclonal,
is prepared in rabbits with homogeneous GFAP isolated from bovine spinal
cords. The antibody is essentially monospecific. It stains only
the astrocytes in formalin-fixed, paraffin-embedded rat brain cerebellum.
Western immunoblots of adult rat spinal cord/cytoskeletal proteins confirmed
the monospecificity. BTI anti-GFAP polyclonal reacts Catalog No: BT-575 BTI anti-Myosin Antibody is prepared in rabbits from bovine uterus myosin. This antisera will react with only heavy chain myosins from smooth muscle and non-muscle sources. Western blot analysis shows that skeletal muscle and cardiac muscle myosins do not cross-react with this antibody. A complete protocol is provided with the product including details on immunofluorescent procedures. Catalog No: BT-564 BTI Non-Muscle Myosin Antibody is prepared in rabbits from Human Platelet Myosin (>95% Pure via PAGE). The non-Muscle Myosin IgG is then purified from rabbit immune serum. The resulting IgG will bind only heavy chain, non-muscle Myosin in vertebrates. It contains no detectable cross-reactivity with any other protein including skeletal, smooth muscle and cardiac myosins. The antibody can be used for Elisa assays, immunoprecipitations and western blots of non-muscle Myosin. It can also be used for the immunohistochemical localization of non-muscle Myosin in vivo (envisioning the blood vasculature system) and in vitro (discriminating between endothelial and smooth muscle cells). A complete protocol is provided with the product including details on immunofluorescent procedures. Catalog No: BT-561 anti-Myosin IgG, Smooth Muscle BTI anti-Smooth Muscle Myosin IgG is prepared in rabbits from bovine tracheal smooth muscle myosin (>98% Pure via PAGE). The anti-Smooth Muscle Myosin IgG is then purified from rabbit immune serum. The resulting IgG binds predominantly heavy chain smooth muscle myosin in vertebrates. It contains no detectable cross-reactivity with skeletal or non-muscle myosin. The antibody can be used for immunohistochemical localization, western blots and for immunoprecipitation studies of smooth muscle myosin. A complete protocol is provided with the product including details on immunofluorescent procedures. Catalog No: BT-562 FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC. Involucrin Immuno-Kit Introduction Human squamous carcinomas of epidermis and oral cavity are defective in involucrin synthesis in culture, and such lesions show corresponding alteration of involucrin staining in vivo. In advanced neoplasms of skin, larynx and bladder, for example, the staining often is patchy, reflecting the evident cellular disorganization. Such patterns are also visible in carcinomas showing squamous differentiation in the lung, endometrium and other locations. In dysplasias of uterine cervix, involucrin staining often is reduced. These results indicate that involucrin staining may be useful in characterizing certain premalignant and malignant lesions, detecting regions of squamous differentiation and determining histogenesis of selected neoplasms. About The Kit The BTI Involucrin Immuno-kit consists of highly specific Involucrin antibody (rabbit) and other reagents to peroxidase stain Involucrin in tissue sections. A complete simple protocol is included with each 100 section kit. The kit should be refrigerated at 4ºC and is stable for 6 months. Kit Components (1 vial each) 1. Anti-Human Involucrin (rabbit)
References 1. Murphy, G.F., Flynn, T.C., Rice, R.H. and Pinkus, G.S. (1984) Involucrin expression in normal and neplastic human skin: a marker for keratinocyte differentiation. J. Invest Dermatol. 82: 453-457. 2. Rice, R.H., Pinkus, G.S., Warhol, M.J. and Antonioli, D.A. (1984) Involucrin: biochemistry and immunohistochemistry In: Advances in immunohistochemistry (S. Sternberg and R. DeLellis, eds.), Masson Publishing, USA pp. 111-125. 3. Said, J.W., Nash,G. Sassoon, A.F., Shintaku, I.P., and Banks-Schlegel, S. (1984) Involucrin in lung tumors. A specific marker for squamous differentiation. Lab Invest. 49: 563-568. 4. Said, J.W., Sassoon, A.F., Shintaku, I.P., and Banks-Schlegel, S., (1984) Involucrin in Squamous and Basal Cell Carcinomas of the Skin: An Immunohistochemical study. J. Invest. Dermatol. 82: 449-452. 5. Warhol, M.J., Antonioli, D.A., Pinkus, G.S., Burke, L. and Rice, R.H. (1982) Immunoperoxidase staining for involucrin, a potential diagnostic aid in cervicovaginal pathology. Human Pathol. 13: 1095-1099. 6. Warhol, N.M., Pinkus, G.S., Rice, R.H. El-Tawil, G.H., Lancaster, W.D., Jenson, A.B. and Kurman, R.J. (1984) Papillomavirus infection of the cervix. III: Relationship of the presence of viral structural proteins to the expression of involucrin. Int.J. Gynecol. Pathol. 3: 71-81. 7. Warhol,, M.J., Rice. R.H., Pinkus, G.S. and Robboy, S.J. (1984) Evaluation of squamous epithelium in adenoacanthoma and adenosquamous carcinoma of the endometrium: Immunoperoxidase analysis of involucrin and keratin localization. Int. J. Gynecol. Pathol 3: 82-91. 8. Kaplan, M.J., Mill, S.E., Rice, R.H., and Johns, M.E. (1984) Involucrin in laryngeal dysplasia. A marker for differentiation. Arch. Of Otolaryngology 110: 713-716. 9. Walts, A.E., Said, J.W., Siegel, M.B. and Banks-Schlegel, S. (1985) Involucrin, a marker of squamous and urothelial differentiation. An immunohistochemical study on its distribution in normal and neoplastic tissues. J. of Pathology 145: 329-340. 10. Sassoon, A.F., Said, J.W. Nash, G.N., Shintaku, I.P. and Banks-Schlegel, S. (1985) Involucrin in intraepithelial and invasive squamous cell carcinomas of the cervix: An immunohistochemical study. Human Pathology 16: 467-470. 11. Rice, R.H. and Thacher, S.M. (1986) Involucrin: A constituent of cross-linked envelopes and marker of squamous maturation. In: biology of the integment Vol. 2 Vertebrates (J. Bereiter-Hahn, A.G. Matoitsy, K.S., Richards (eds.) Springer-Verlag. Berlin Heidelberg, Germany. 752-761. 12. Smoller, B.R., Kwan, T.H., Said, J.W. and Banks-Schlegel, S. (1986) Keratoacanthoma and squamous cell carcinoma of the skin: Immunohistochemical localization of involucrin and keratin proteins. J. Am. Acad. Dermatology 14: 226-234. 13. Michael S., Schmidt, R., Robinson, S.M., Schroot, B., and Reichert, U. (1987) Identification and subcellular distribution of cornified envelope precursor proteins in the transformed human keratinocyte line KV-K14. J. Invest. Dermatol. 88: 301-305. 14. Mansbridge, J.N. and Knapp, A.M. (1987) Changes in keratinocyte maturation during wound healing. J. Invest. Dermatol. 89: 253-263. 15. Greenberg,
A.M. (1987) Involucrin expression as a marker for neoplastic changes in
oral mucosa. J. of Oral & Maxillofacial Surgery 45, No. 11 (Abstract).
Involucrin Immuno-kit
Refrigerate at 4ºC.
Do Not Freeze. FOR RESEARCH USE ONLY. NOT
FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC. |
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